Skip to main content
. 2016 Nov 15;11(11):e0166653. doi: 10.1371/journal.pone.0166653

Table 1. Details of primers, probe, nucleotide sequences, gene positions product sizes in PCR assays and sequencing of rickettsial genes [2].

Gene Primers and probe Nucleotide sequence (5´to 3`) Product size (bp)
gltA CS-F TCG CAA ATG TTC ACG GTA CT 74
CS-R TCG TGC ATT TCT TTC CAT TGT G
CD-P 6-FAM-TGC AAT AGC AAG AAC CGTAGG CTG GAT G—BBQ-1 Probe
ompB Rc.rompB.4362p GTC AGC GTT ACT TCT TCG ATG C 475
Rc.rompB.4,836n CCG TAC TCC ATC TTA GCA TCA G
Rc.rompB.4,496p CCA ATG GCA GGA CTT AGC TAC T 267
Rc.rompB.4,762n AGG CTG GCT GAT ACA CGG AGT AA
17kDa Rr17kDa.61p GCT CTT GCA ACT TCT ATG TT 434
Rr17kDa.492n CAT TGT TCG TCA GGT TGG CpG
gltA RH314 AAA CAG GTT GCT CAT CAT TC 857
CSF-R AAG TAC CGT GAA CAT TTG CGA
CS-Ric-R CAG TGA ACA TTT GCG ACG GTA 852
CS535d GCA ATG TCT TAT AAA TAT TC Sequencing primer

FAM = fluorescein amidite.