Table 1. Details of primers, probe, nucleotide sequences, gene positions product sizes in PCR assays and sequencing of rickettsial genes [2].
Gene | Primers and probe | Nucleotide sequence (5´to 3`) | Product size (bp) |
---|---|---|---|
gltA | CS-F | TCG CAA ATG TTC ACG GTA CT | 74 |
CS-R | TCG TGC ATT TCT TTC CAT TGT G | ||
CD-P | 6-FAM-TGC AAT AGC AAG AAC CGTAGG CTG GAT G—BBQ-1 | Probe | |
ompB | Rc.rompB.4362p | GTC AGC GTT ACT TCT TCG ATG C | 475 |
Rc.rompB.4,836n | CCG TAC TCC ATC TTA GCA TCA G | ||
Rc.rompB.4,496p | CCA ATG GCA GGA CTT AGC TAC T | 267 | |
Rc.rompB.4,762n | AGG CTG GCT GAT ACA CGG AGT AA | ||
17kDa | Rr17kDa.61p | GCT CTT GCA ACT TCT ATG TT | 434 |
Rr17kDa.492n | CAT TGT TCG TCA GGT TGG CpG | ||
gltA | RH314 | AAA CAG GTT GCT CAT CAT TC | 857 |
CSF-R | AAG TAC CGT GAA CAT TTG CGA | ||
CS-Ric-R | CAG TGA ACA TTT GCG ACG GTA | 852 | |
CS535d | GCA ATG TCT TAT AAA TAT TC | Sequencing primer |
FAM = fluorescein amidite.