Table 1.
Representative proteins uniquely labeled by 1, 2 or 3 in HEK293T cells and identified by mass spectrometry. The number of total matched peptides spectra from two independent experiments is listed. SCD1 inhibitor (CAY) is used to block desaturation of 1 in cells.
| Symbol | Protein name | 1 + CAY | 2 | 3 |
|---|---|---|---|---|
| F1712 | Protein FAM171A2 | 7/6 | 3/0 | 0/0 |
| GNA12 | G- protein subunit alpha-12 | 6/6 | 0/0 | 0/0 |
| EFNA5 | Ephrin-A5 | 5/6 | 0/0 | 0/0 |
| LRC57 | Leucine-rich repeat-containing protein 57 | 4/7 | 1/0 | 0/0 |
| GAPR1 | Golgi-associated plant pathogenesis-related protein 1 | 5/5 | 1/1 | 0/0 |
| NDUF4 | NADH dehydrogenase 1 alpha factor 4 | 2/6 | 0/1 | 0/0 |
| HS904 | Putative heat shock protein HSP 90-alpha A4 | 7/0 | 0/0 | 0/0 |
| L12R1 | Loss of heterozygosity 12 chromosomal region 1 protein | 2/5 | 0/0 | 0/0 |
| DCNL3 | DCN1-like protein 3 | 2/5 | 0/0 | 0/0 |
| GPC1 | Glypican-1 | 3/3 | 0/0 | 0/0 |
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| ABHD2 | Abhydrolase domain-containing protein 2 | 0/0 | 10/11 | 6/0 |
| MASU1 | Mitochondrial ribosomal large subunit protein 1 | 0/0 | 4/6 | 0/0 |
| SFT2A | Vesicle transport protein SFT2A | 0/0 | 5/2 | 1/0 |
| ATLA3 | Atlastin-3 | 0/2 | 5/2 | 3/0 |
| UFM1 | Ubiquitin-fold modifier 1 | 0/0 | 2/4 | 0/2 |
| DDX20 | RNA helicase DDX20 | 1/0 | 4/2 | 0/0 |
| SPRE | Sepiapterin reductase | 1/0 | 2/4 | 0/0 |
| WNT11 | Protein Wnt-11 | 0/0 | 3/2 | 0/0 |
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| MYOF | Myoferlin | 0/0 | 0/0 | 30/7 |
| SAFB1 | Scaffold attachment factor B1 | 0/0 | 0/2 | 3/4 |
| ELOB | Transcription elongation factor B polypeptide 2 | 1/0 | 0/0 | 4/3 |
| OCTC | Peroxisomal carnitine O-octanoyltransferase | 2/0 | 0/1 | 7/0 |
| C9JG07 | Vacuolar protein sorting-associated protein 8 | 0/0 | 0/0 | 3/3 |
| IMDH1 | Inosine-5′-monophosphate dehydrogenase 1 | 0/0 | 0/0 | 3/3 |
| AMFR | E3 ubiquitin-protein ligase AMFR | 1/0 | 1/1 | 4/2 |