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Genomically recoded organisms (GROs), strain engineering and in vitro nsAA translation. A genomically recoded organism reassigns the TAG stop codon to an open sense coding channel for enhanced nsAA amber suppression. Ribosomal engineering promotes other classes of codon suppression. These engineered strains can be used in vivo and via cell lysate, to develop in vitro translation systems with open coding channels for the production of nsAA-containing proteins.
