Fig. 7.
HOCl and dopamine at 10−8M concentrations produce aggregates. Aggregates were produced by reacting HOCl and dopamine, at final concentrations of 10 and 20 nM respectively, in PBS at 22 °C for periods of up to 72 h. Panel A depicts the aggregates produced in either PBS or chlorodopamine mixtures as a function of reaction time and expressed as aggregates per s. The data in panel A is a summation of distributions of the type shown in panels B and C. These values are shown as the mean and SEM of 14 experiments (A). Panels B and C depict the distributions arising from the reaction of HOCl and dopamine for five (B) and 24 (B) h; the black bars in these panels represent the mean of 14 experiments that varied no more than 10% of the mean for each value of Forward Scatter. The inserts exhibit expanded views (Forward Scatter: 295–550) from the corresponding panels. Note: the abscissa and ordinate scales vary between the panels and inserts.