Skip to main content
. 2016 Jun 24;8(3):145–154. doi: 10.1038/ijos.2016.16

Table 1. Primer sequences and product size of RT-PCR.

Protein Primer Sequence (5'→3') Product size/bp
K3 Type II intermediate filaments in mammalian cells during differentiation and stratified epithelial cells GACAATAATCGTTCCCTGGTTGCGGTAGGTGGCGATCT 434
K14 Type I intermediate filaments in inmature cells of stratified epithelial cells ACTACCTGCAGCCGCCAGTTCAGTTCTTGGTGCGAAGGAC 1 417
p63 Tumour suppressor gene in inmature cells of stratified epithelial cells CAGACTCAATTTAGTGAGGTGCCCCAACCATGAGCT 440
Pax7 Paired box transcription factor family member involved in maintaining proliferation and preventing differentiation in skeletal muscle progenitor cells ATCCGGCCCTGTGTCATCTCCACGCGGCTAATCGAACTCA 278
CD34 Hematopoietic stem cell marker. CD34 expressed in quiescent adult skeletal muscle satellite cells AATCTAGCCCAGTCTGAGGTCTTTCGGGAATAGCTCTGGT 174
Desmin Type III intermediate filament in skeletal, smooth and cardiac muscle tissue TGCAGGAGCTCAATGACCTCGATGCGAGCTAGTGTG 337
MyoD A protein with a key role in regulating muscle differentiation GCTCGCGAGGATGAGCATGTATGGCGTTGCGCAGGATCTC 239
GAPDH Glyceraldehyde-3-phosphate dehydrogenase, internal control ACCACAGTCCATGCCATCACTCCACCACCCTGTTGCTGTA 452

RT-PCR, reverse transcriptase polymerase chain reaction.