Table 1. Primer sequences and product size of RT-PCR.
| Protein | Primer | Sequence (5'→3') | Product size/bp |
|---|---|---|---|
| K3 | Type II intermediate filaments in mammalian cells during differentiation and stratified epithelial cells | GACAATAATCGTTCCCTGGTTGCGGTAGGTGGCGATCT | 434 |
| K14 | Type I intermediate filaments in inmature cells of stratified epithelial cells | ACTACCTGCAGCCGCCAGTTCAGTTCTTGGTGCGAAGGAC | 1 417 |
| p63 | Tumour suppressor gene in inmature cells of stratified epithelial cells | CAGACTCAATTTAGTGAGGTGCCCCAACCATGAGCT | 440 |
| Pax7 | Paired box transcription factor family member involved in maintaining proliferation and preventing differentiation in skeletal muscle progenitor cells | ATCCGGCCCTGTGTCATCTCCACGCGGCTAATCGAACTCA | 278 |
| CD34 | Hematopoietic stem cell marker. CD34 expressed in quiescent adult skeletal muscle satellite cells | AATCTAGCCCAGTCTGAGGTCTTTCGGGAATAGCTCTGGT | 174 |
| Desmin | Type III intermediate filament in skeletal, smooth and cardiac muscle tissue | TGCAGGAGCTCAATGACCTCGATGCGAGCTAGTGTG | 337 |
| MyoD | A protein with a key role in regulating muscle differentiation | GCTCGCGAGGATGAGCATGTATGGCGTTGCGCAGGATCTC | 239 |
| GAPDH | Glyceraldehyde-3-phosphate dehydrogenase, internal control | ACCACAGTCCATGCCATCACTCCACCACCCTGTTGCTGTA | 452 |
RT-PCR, reverse transcriptase polymerase chain reaction.