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. 2016 Jul 19;24(9):1644–1654. doi: 10.1038/mt.2016.120

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Copyright © 2016 Official journal of the American Society of Gene & Cell Therapy

This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-sa/4.0/

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Transient expression of HGF was controlled by the Dox concentration. (a) Diagram of the inducible HGF-expression system. The In-fusion system was used to combine the TetOn and HGF systems in the vector. (b) Western blotting (WB) analysis of HGF expression by 293T cells at 48 hours post-Dox treatment. (c) The level of HGF expression by 293T cells was dependent on the Dox concentration. (d) Diagram of the vector constructed for integration into the PPPR12C site of chromosome 19 using the In-fusion system. (e) WB analysis of HGF expression via the integration vector in ADSCs cultured for 48 hours and the level of HGF in the medium that they conditioned as well as that (f) in the hUCB-MSC conditioned medium.