Figure 5.

The effect of pGag codelivery during prophylactic anti-GP100 immunization on the antitumor activity. (a) Experimental plan. C57BL/6 mice were immunized in a regimen of one prime and two boosts at a 2-week interval with the antigenic GP100 plasmid combined or not with pGag. Two weeks after the last vaccination, they were challenged with B16F10-OVA cells and tumor growth and mouse survival were monitored. (b) Survival rates monitoring after challenge. The asterisks indicate significant differences compared with naive mice (*P < 0.05) (comparison of survival curves, Mantel–Cox test). (c) Tumor growth follow-up after challenge in mice immunized with pGP100 and pGP100 combined with pGag. The results are expressed as mean − SEM (n = 6). The asterisks show significant differences between groups (*P < 0.05) (analysis of variance, Dunnett's post-hoc test).