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. 2016 Aug 9;24(9):1675–1685. doi: 10.1038/mt.2016.134

Figure 5.

Figure 5

In vitro epitope presentation (MHC I and MHC II-restricted) assay. Processing of Z13OVACD8CD4, Z14OVACD8CD4, Z15OVACD8CD4, and Z18OVACD8CD4 by BMDCs and antigen presentation to OVA257–264 specific, MHC class I restricted (H-2Kb) OT-I T cells (a) or to OVA323-339 specific, MHC class II restricted (H-2IAb) OT-II T cells (b) in vitro. CFSE-labeled splenocytes from OT-I or OT-II mice were cocultured for 4 days alone with mature BMDCs that had been loaded with each ZEBRA CPP construct. Proliferation was assessed by CFSE dilution analyzed by flow cytometry, gating on live CD8 (OT-I cells, a) or CD4 (OT-II cells, b) T cells. As positive control, BMDCs were pulsed for 1 hour with 5 µmol/l peptide. Data from one experiment representative of three independent experiments. BMDC, bone marrow derived DCs; CPP, cell penetrating peptide; MHC, ; CFSE, carboxyfluorescein diacetate succinimidyl ester; OVA, ovalbumin.