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. 2016 Aug 30;24(9):1665–1674. doi: 10.1038/mt.2016.142

Figure 1.

Figure 1

Sialic acid Neu5GC on in vitro propagated mesenchymal stem cells (MSCs) triggers complement-mediated damage. (a) MSCs from different donors (640, 678, 741, 742, and 784) were evaluated for the presence of Neu5GC by flow cytometry after staining with anti-Neu5GC IgY (solid lines) or control IgY (dotted lines). (b) Left panel: Levels of Neu5GC on MSCs cultured for 7 days in the absence or presence of Neu5AC. Dotted line and solid line: MSCs incubated with Neu5AC and stained with control IgY (dotted line) or with anti-Neu5GC IgY (solid line). Dashed lines and shaded areas, MSCs cultured in the absence of Neu5AC and stained with control IgY (dashed line) or anti-Neu5GC IgY (shaded area). middle panels: Levels of IgM and IgGs on these MSCs after incubation with 30% serum for 30 minutes. Right panel, levels of C3b deposition on MSCs cultured in the absence or presence of Neu5AC after incubating them with 30% serum for 30 minutes in GVB++. (c) Complement-mediated cell damage when MSCs cultured in the presence (black bars) or absence (gray bars) of Neu5AC were incubated with 20 or 30% normal human serum in GVB++. The data are the combined results for three individual experiments ± SEM. *P < 0.05.