Figure 4.

Assessment of muscle pathology. (a) Representative pictures of gastrocnemius muscle cross-sections. Gastrocnemius muscles were dissected from P12 mice and cross-sections were stained with hematoxylin and eosin and analyzed by microscopy. (b) Mean fiber size was quantified using the Metamorph (Version 6.2r4) software. Data are presented as mean ± SEM. (c) Muscle fiber distribution was analyzed for each group. Both E1^MO and E1^MOv11 had significantly fewer small fibers and significantly higher percent of larger fibers than the SMA noninjected controls. Significance was calculated using Student's t-test, where ***denotes P ≤ 0.001. SMA, spinal muscular atrophy.