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. 2016 Aug 9;24(9):1655–1664. doi: 10.1038/mt.2016.147

Figure 1.

Figure 1

Millimolar adenosine suppresses T-cell alloactivation and proliferation. (a) FACS analysis showing percent CD25+ T cells in culture after stimulation of healthy donor lymphocytes with HLA-mismatched recipient DC over 8 days in the presence or absence of various doses of adenosine on days 1, 2, and 5 after establishing cocultures. (b) CFSE-labeled donor lymphocytes were cocultured with HLA-mismatched recipient DCs for 10 days. Cultures were treated with 2 mmol/l adenosine on day 2 or 5 only, or days 1, 2, and 5 after establishing mMLR. Two days after the final treatment, CFSE dye dilution in T cells was determined as a measure of cell proliferation by flow cytometry. Results shown in Figure 1a and 1b are the mean of 6 HLA-mismatched donor–recipient pairs. (c) Lymphocytes from three healthy donors were stimulated with anti-CD3/CD28 beads and treated with 2 mmol/l adenosine on days 1, 2, and 5 (+Ado) or not treated (No Ado). Two days after the final treatment cultures were stained with vivid and viability was determined by flow cytometry. Results shown are representative of three donors.