Figure 10. Effect of trapping on long-term bacterial function.

(a) Fluorescence plots of real-time PCR on tRNA^met of untreated (white) and trapped (black) bacteria at 24 h (circle), 48 h (square) and 72 h (triangle). (b) Analysis of tRNA^met relative expression level in term of Cq (cycle of fluorescence over threshold 500 units) between untreated (white) and trapped (black) bacteria after experiment from 24 to 72 h. (c) Fluorescence signal from green fluorescence protein between untreated (white) and trapped (black) bacteria after experiment from 0 to 72 h. Results are mean ± SEM (n = 3 independent experiments).