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. 2016 Nov 17;6:36736. doi: 10.1038/srep36736

Figure 9. Kim1 is ectopically expressed initially in Six2creFrs2αKO non-dilated proximal tubular cells.

Figure 9

(A) Quantitative PCR analysis reveals increases in mutant kidney Kim1 mRNA expression versus controls starting at P7 and worsening at P21. (B) Western blot analysis at P21 confirms a significant increase in Kim1 protein in mutant kidneys (M1-3) versus controls (C1–3) (cropped blots are shown as indicated by the lines- these blots were run under the same experimental conditions). (C,D) Co-immunofluorescence for Kim1 (red) and LTL (green) at P7 reveals that while controls display no Kim1 expression (C), subsets of mutant non-dilated LTL-positive proximal cells ectopically express Kim1 (D, arrowheads), whereas mutant LTL-positive cysts (D, asterisks) do not. (E,F) Co-immunofluorescence for Kim1 (red) and LTL (green) at P21 again shows no obvious Kim1 expression in control kidneys (E), whereas many minimally-dilated and overtly cystic LTL-positive mutant cells ectopically express Kim1 (F, arrowhead and arrow, respectively). (A,B) scale bar = (C–F) scale bar = 25 μm.