. Author manuscript; available in PMC: 2017 Nov 1.

Published in final edited form as: J Immunol. 2016 Oct 5;197(9):3680–3694. doi: 10.4049/jimmunol.1600719

Table 1.

mRNA for MASPs, MAp44 and Df in the liver and in adipose tissue in WT mice with CAIA¹

mRNA	Tissue	siRNAs scramble²	siRNAs MASP-1/3²	siRNAs Df²
MASP-1	Liver	555.9 ± 92.4	213.5 ± 99.3	359.4 ± 25.4
P	0.034^*		0.04	0.07
Decrease			62%	35%
MASP-1	Adipose	2.7 ± 0.94	2.12 ± 0.37	2.75 ± 0.47
p	0.53^*
MASP-3	Liver	99.97 ± 17.06	41.62 ± 12.4	65.81 ± 6.74
p	0.042^*		0.04	0.09
Decrease			58%	34%
MASP-3	Adipose	2.11 ± 0.64	1.70 ± 0.31	1.53 ± 0.18
p	0.64^*
MAp44	Liver	23.9 ± 3.1	49.41 ± 8.95	40.5 ± 4.0
p	0.019^*		0.01	0.02
Increase			52%	41%
Map44	Adipose	0.42 ± 0.29	0.95 ± 0.28	0.49 ± 0.07
p	0.36^*		0.068	0.28
Df	Liver	0.85 ± 0.23	0.47 ± 0.18	0.97 ± 0.34
p	0.44^*
Df	Adipose	8632.2 ± 824.3	6290.2 ± 508.3	4076.0 ± 1042.1
p	0.009^*		0.06	0.008
Decrease			27%	53%

Data are expressed in pg/ng 18s rRNA with mean ± SEM based on the indicated number of mice (n).

Mice with injected three times i.e. at day −5, day 0 and at day 3 subcutaneously (SQ) with scrambled siRNAs (n = 5), MASP-1/3 siRNAs (n = 5) and Df siRNAs (n =5). CAIA was also induced in these mice as mentioned in the Methods section. At day 10, liver and adipose tissue (visceral fat) from all CAIA was removed and analyzed for mRNAs for MASP-1, MASP-3 and Df. All p-values for different mRNAs in mice treated SQ either with siRNAs MASP-1/3 or siRNAs Df were compared with the corresponding values of WT mice treated with SQ treated scrambled siRNAs. The percent (%) decrease in mRNA levels in mice treated SQ with siRNAs MASP-1/3 or siRNAs Df in each column has been shown compared with mice treated SQ with scrambled siRNAs. The mRNA levels for MASP-1 or MASP-3 were below the detection limits in the adipose tissue and similarly the mRNA levels for Df were below the detection limits in the liver of mice with CAIA injected SQ with siRNAs for scramble, MASP-1/3 or Df. p values were compared between CAIA mice treated either with scrambles siRNA and siRNAs MASP-1/3 or siRNA Df. p < 0.05 were considered statistically significant.

p value among all siRNA treatment groups using ANOVA