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. Author manuscript; available in PMC: 2017 Oct 3.
Published in final edited form as: Curr Protoc Neurosci. 2016 Oct 3;77:9.54.1–9.54.10. doi: 10.1002/cpns.14

Figure 1.

Figure 1

(A, B) Downstream smoke is bubbled through 35 ml of saline solution in a disposable tube. (C). Smoke is drawn by an experimenter-operated syringe over 2 seconds, every 30 seconds.