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. 2016 Jul 17;34(11):2744–2757. doi: 10.1002/stem.2442

Figure 6.

Figure 6

Selective knockout of CXCR4 in smooth muscle cells (SMCs) reduces intimal hyperplasia (IH) in wire‐injured mouse femoral arteries. SM22‐driven conditional CXCR4 knockout model was created and wire injury was performed in wild type and CXCR4 knockout mice, as described in Methods. (A–E): Representative H&E stained femoral arterial sections in uninjured (A), injured wild type (B) or injured knockout mice (C). (D) and (E) are enlarged views of the boxed areas in (B) and (C). (F–J): CXCR4 immunostaining in uninjured (F), injured wild type (G), or injured knockout mice (H). (I) and (J) are enlargement of the boxed regions from (G) and (H). (K‐M): SDF‐1α immunostaining in uninjured (K), injured wild type (L), or injured knockout mice (M). (N–O): SMA immunostaining in injured wild type (N) or injured knockout mice (O). (P–R): Smad3 immunostaining in uninjured (P), injured wild type (Q), or injured knockout mice (R). (S): IH (intima/media area ratio), lumen area, and media area were quantified as described in Methods. *p < .05 compared to wild type; n = 12‐13. Scale bar = 50 µm. Abbreviation: SDF‐1, stromal cell‐derived factor‐1.