Table I.
The Effects of Varied Loop Size on the Melting Temperature (Tm) of the Complexes of Circular Oligonucleotides with d(A)12 and with a 36mer Containing the d(A)12 Sequencea
| circle | loop size (nt) |
Tm of complex (°C)
|
|
|---|---|---|---|
| 12mer substrate | 36mer substrate | ||
|
|
3 | 49.2 | |
|
|
4 | 54.9 | 49.7 |
|
|
5 | 58.6 | 56.2 |
|
|
6 | 55.8 | 52.3 |
|
|
10 | 54.1 | |
See text for 36mer sequence and for experimental conditions.