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Comparison of Binding Affinities of Nicked Circles (Linear Oligomers) with That of a Covalently Closed Circlea
| entry | complex | Tm (°C) |
|---|---|---|
| 1 |
|
43.8 |
| 2 |
|
46.4 |
| 3 |
|
46.6 |
| 4 |
|
54.9 |
| 5 |
|
62.3 |
Melting temperatures (Tm) were measured at 260 nm, pH 7.0, for the complexes with the complement 5′-d(AAGAAAAGAAAG). “P” denotes a 5′ phosphate group.
