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. 2016 Nov 1;23(11):1294–1306. doi: 10.5551/jat.34082

Fig. 3.

Fig. 3.

Intermedin1–53 attenuated endoplasmic reticulum stress (ERS) induced by homocysteine (Hcy) in vivo and in vitro. Western blot analysis of protein levels (a, b) of ERS hallmarkers such as glucose-regulated protein 78 (GRP78) and GRP94, activating transcription factor 6 (ATF6), phosphorylated inositol-requiring enzyme 1α (p-IRE1α), spliced-Xbox-binding protein-1 (s-XBP-1), phosphorylated protein kinase receptor-like ER kinase (p-PERK), phosphorylated eukaryotic translation initiation factor 2α (p-eIF2α) and ATF4 in the myocardium of ApoE-/- mice in vivo. Western blot analysis of protein levels (c, d) of ERS hallmarkers such as ATF6, ATF4, GRP94, GRP78, p-eIF2α, p-PERK, p-IRE1α, and s-XBP-1 in rat cardiac fibroblasts in vitro. Glyceraldehyde-3-phosphate dehydrogenase was a loading control. Data are mean ± SD, n = 3 in each group; *P < 0.05 and **P < 0.01 versus Con. #P < 0.05 and ##P < 0.01 versus Hcy.