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. Author manuscript; available in PMC: 2017 Apr 10.
Published in final edited form as: Nat Chem Biol. 2016 Oct 10;12(12):998–1000. doi: 10.1038/nchembio.2180

Figure 3. Modulation of serine ADP-ribosylation marks on core histones upon DNA damage.

Figure 3

(a) Schematic representation of the SILAC-based strategy to quantify core histone marks upon 10 minutes of 2 mM H2O2-induced DNA damage. (b) Western blot analysis of total protein poly-ADP-ribosylation levels prior to mixing light and heavy lysates from each SILAC experiment. Anti-GAPDH was used as a loading control. See Supplementary Fig. 10 for original images. (c) Log transformed Heavy/Light SILAC ratios from the forward and Light/Heavy SILAC ratios from the reverse experiment were plotted against each other. Each point represents a unique histone mark.