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. 2016 Jul 8;44(9):2654–2666. doi: 10.1111/ejn.13291

Figure 4.

Figure 4

Dendritic atrophy of ventral hippocampal pyramidal neurons in GF mice. (A, B) Representative photomicrograph of a Golgi‐stained pyramidal neuron in the ventral hippocampus (A) and resulting computer‐assisted morphometric reconstruction (B). Scale bars = 50 μm. (C) Representative photomicrographs of ventral hippocampal pyramidal neuron dendritic spine density in CC and GF mice. Scale bar = 10 μm. (D, E) The dendrites of ventral hippocampal pyramidal neurons of GF mice were significantly shorter (D) and had less branching compared to controls (E). (F) In GF mice, Sholl analysis revealed a retraction in intermediate dendrites (160–380 μm from the soma). (G, H) The spines on basilar dendrites were thinner in GF mice (G), but there was no significant difference in the length of spines between groups (H). (I) GF mice had less spines on all portions of the dendritic arbour of ventral hippocampal pyramidal neurons compared to CC animals. (J, K, L) This reduction in spine density was due to a decrease in stubby spines (K) and mushroom spines (L), but not of thin spines (J). CC, n = 5; GF, n = 6. *P ≤ 0.05, **P < 0.01.