Figure 5.

Reduced branching of dentate granule cells in GF mice. (A, B) Representative photomicrograph of a Golgi‐stained dentate granule cell in the dorsal hippocampus (A) and resulting computer‐assisted morphometric reconstruction (B). Scale bars = 50 μm. (C) Representative photomicrographs of granule cell spine density in CC and GF mice. Scale bar = 10 μm. (D, E) The dendrites of granule cell pyramidal neurons of GF mice did not differ in length (D), but had less branching compared to controls (E). (F) In GF mice, Sholl analysis revealed a loss of dendritic complexity; however, post hoc comparisons revealed no statistically significant distances in which this reduction in branching occurred. (I, J, K, L) There was no difference in the density of all (I), thin (J), stubby (K) or mushroom (L) spines in GF versus CC mice. n = 6 for both groups. *P ≤ 0.05.