Skip to main content
. 2016 Aug 25;46(10):2340–2351. doi: 10.1002/eji.201646446

Figure 2.

Figure 2

Reactivity of IgA+ memory B‐cell antibodies against vaccines, viral pathogens, and commensal bacteria. The reactivity of recombinant IgA+ and IgG+ memory antibodies against selected vaccines, mucosa‐tropic viruses, and commensal bacteria was determined by ELISA. In total, 251 recombinant IgA (from four healthy individuals; n = 59 for hd1, n = 47 for hd2, n = 67 for hd3, and n = 78 for hd4), and 61 IgG monoclonal antibodies (from hd2 and hd4 donors, n = 28 and n = 33, respectively) were produced and tested. Antibodies were tested in triplicate and in two independent experiments. Averaged data from one representative experiment are shown. (A) Flu, influenza virus; DTP, diphtheria‐tetanus‐polio; MMR, measles‐mumps‐rubella; HBV, hepatitis B virus. (B) Adeno, adenovirus type 5; Rota, rotavirus; Rhino, rhinovirus type 1A; HIV‐1. (C) Selected commensal bacteria. Dotted black lines indicate the reactivity of the polyreactive control antibody ED38 38. Dotted green line indicates the average reactivity of the negative control antibody mGO53 8 against the tested antigens. Polyreactive antibodies reacting non‐specifically with the different antigens tested are shown in red.