Table 1.
Sequences of DNA substrates used in the study
Substrates used in DNA binding assays: |
1. 11/28-mer non-G4 DNA |
5'-ATCCTCCCCTA-3′ |
3'-TAGGAGGGGATGGGTCGTATCAGTGTAT-/FAM/-5′ |
2. 11/28-mer G4 DNA a |
5'-ATCCTCCCCTA-3′ |
3'-TAGGAGGGGATGGGTGGGATGGGTGGGT-/FAM/-5′ |
Substrates used in DNA pol activity assays: |
1. 13/42-mer non-G4 DNA |
5′-/FAM/-TTTGCCTCGAGCCAGC-3′ |
3′-CGGAGCTCGGTCGGCGTCTGCGTGGGTACCAGTTGTAGAGTG-5′ |
2. 14/42-mer non-G4 DNA |
5′-/FAM/-TTTGCCTCGAGCCAGCC-3′ |
3′-CGGAGCTCGGTCGGCGTCTGCGTGGGTACCAGTTGTAGAGTG-5′ |
3. 18/42-mer non-G4 DNA |
5′-/FAM/-TTTGCCTCGAGCCAGCCGCAG-3′ |
3′-CGGAGCTCGGTCGGCGTCTGCGTGGGTACCAGTTGTAGAGTG-5′ |
4. 19/42-mer non-G4 DNA |
5′-/FAM/-TTTGCCTCGAGCCAGCCGCAGA-3′ |
3′-CGGAGCTCGGTCGGCGTCTGCGTGGGTACCAGTTGTAGAGTG-5′ |
5. 20/42-mer non-G4 DNA |
5′-/FAM/-TTTGCCTCGAGCCAGCCGCAGAC-3′ |
3′-CGGAGCTCGGTCGGCGTCTGCGTGGGTACCAGTTGTAGAGTG-5′ |
6. 21/42-mer non-G4 DNA |
5′-/FAM/-TTTGCCTCGAGCCAGCCGCAGACG-3′ |
3′-CGGAGCTCGGTCGGCGTCTGCGTGGGTACCAGTTGTAGAGTG-5′ |
7. 22/42-mer non-G4 DNA |
5′-/FAM/-TTTGCCTCGAGCCAGCCGCAGACGC-3′ |
3′-CGGAGCTCGGTCGGCGTCTGCGTGGGTACCAGTTGTAGAGTG-5′ |
8. 23/42-mer non-G4 DNA |
5′-/FAM/-TTTGCCTCGAGCCAGCCGCAGACGCA-3′ |
3′-CGGAGCTCGGTCGGCGTCTGCGTGGGTACCAGTTGTAGAGTG-5′ |
9. 13/42-mer G4 DNA |
5′-/FAM/-TTTGCCTCGAGCCAGC-3′ |
3′-CGGAGCTCGGTCGGCGTCTGCGTGGGTGGGATGGGTGGGAGT-5′ |
10. 14/42-mer G4 DNA |
5′-/FAM/-TTTGCCTCGAGCCAGCC-3′ |
3′-CGGAGCTCGGTCGGCGTCTGCGTGGGTGGGATGGGTGGGAGT-5′ |
11. 18/42-mer G4 DNA |
5′-/FAM/-TTTGCCTCGAGCCAGCCGCAG-3′ |
3′-CGGAGCTCGGTCGGCGTCTGCGTGGGTGGGATGGGTGGGAGT-5′ |
12. 19/42-mer G4 DNA |
5′-/FAM/-TTTGCCTCGAGCCAGCCGCAGA-3′ |
3′-CGGAGCTCGGTCGGCGTCTGCGTGGGTGGGATGGGTGGGAGT-5′ |
13. 20/42-mer G4 DNA |
5′-/FAM/-TTTGCCTCGAGCCAGCCGCAGAC-3′ |
3′-CGGAGCTCGGTCGGCGTCTGCGTGGGTGGGATGGGTGGGAGT-5′ |
14. 21/42-mer G4 DNA |
5′-/FAM/-TTTGCCTCGAGCCAGCCGCAGACG-3′ |
3′-CGGAGCTCGGTCGGCGTCTGCGTGGGTGGGATGGGTGGGAGT-5′ |
15. 22/42-mer G4 DNA |
5′-/FAM/-TTTGCCTCGAGCCAGCCGCAGACGC-3′ |
3′-CGGAGCTCGGTCGGCGTCTGCGTGGGTGGGATGGGTGGGAGT-5′ |
16. 23/42-mer G4 DNA |
5′-/FAM/-TTTGCCTCGAGCCAGCCGCAGACGCA-3′ |
3′-CGGAGCTCGGTCGGCGTCTGCGTGGGTGGGATGGGTGGGAGT-5′ |
Tetrad-associated guanines are shown in bold with the G22 guanine underlined.