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. Author manuscript; available in PMC: 2017 Sep 20.
Published in final edited form as: Biochemistry. 2016 Sep 7;55(37):5218–5229. doi: 10.1021/acs.biochem.6b00374

Table 1.

Sequences of DNA substrates used in the study

Substrates used in DNA binding assays:
1. 11/28-mer non-G4 DNA
5'-ATCCTCCCCTA-3′
3'-TAGGAGGGGATGGGTCGTATCAGTGTAT-/FAM/-5′
2. 11/28-mer G4 DNA a
5'-ATCCTCCCCTA-3′
3'-TAGGAGGGGATGGGTGGGATGGGTGGGT-/FAM/-5′
Substrates used in DNA pol activity assays:
1. 13/42-mer non-G4 DNA
5′-/FAM/-TTTGCCTCGAGCCAGC-3′
    3′-CGGAGCTCGGTCGGCGTCTGCGTGGGTACCAGTTGTAGAGTG-5′
2. 14/42-mer non-G4 DNA
5′-/FAM/-TTTGCCTCGAGCCAGCC-3′
    3′-CGGAGCTCGGTCGGCGTCTGCGTGGGTACCAGTTGTAGAGTG-5′
3. 18/42-mer non-G4 DNA
5′-/FAM/-TTTGCCTCGAGCCAGCCGCAG-3′
    3′-CGGAGCTCGGTCGGCGTCTGCGTGGGTACCAGTTGTAGAGTG-5′
4. 19/42-mer non-G4 DNA
5′-/FAM/-TTTGCCTCGAGCCAGCCGCAGA-3′
    3′-CGGAGCTCGGTCGGCGTCTGCGTGGGTACCAGTTGTAGAGTG-5′
5. 20/42-mer non-G4 DNA
5′-/FAM/-TTTGCCTCGAGCCAGCCGCAGAC-3′
    3′-CGGAGCTCGGTCGGCGTCTGCGTGGGTACCAGTTGTAGAGTG-5′
6. 21/42-mer non-G4 DNA
5′-/FAM/-TTTGCCTCGAGCCAGCCGCAGACG-3′
    3′-CGGAGCTCGGTCGGCGTCTGCGTGGGTACCAGTTGTAGAGTG-5′
7. 22/42-mer non-G4 DNA
5′-/FAM/-TTTGCCTCGAGCCAGCCGCAGACGC-3′
    3′-CGGAGCTCGGTCGGCGTCTGCGTGGGTACCAGTTGTAGAGTG-5′
8. 23/42-mer non-G4 DNA
5′-/FAM/-TTTGCCTCGAGCCAGCCGCAGACGCA-3′
    3′-CGGAGCTCGGTCGGCGTCTGCGTGGGTACCAGTTGTAGAGTG-5′
9. 13/42-mer G4 DNA
5′-/FAM/-TTTGCCTCGAGCCAGC-3′
    3′-CGGAGCTCGGTCGGCGTCTGCGTGGGTGGGATGGGTGGGAGT-5′
10. 14/42-mer G4 DNA
5′-/FAM/-TTTGCCTCGAGCCAGCC-3′
    3′-CGGAGCTCGGTCGGCGTCTGCGTGGGTGGGATGGGTGGGAGT-5′
11. 18/42-mer G4 DNA
5′-/FAM/-TTTGCCTCGAGCCAGCCGCAG-3′
    3′-CGGAGCTCGGTCGGCGTCTGCGTGGGTGGGATGGGTGGGAGT-5′
12. 19/42-mer G4 DNA
5′-/FAM/-TTTGCCTCGAGCCAGCCGCAGA-3′
    3′-CGGAGCTCGGTCGGCGTCTGCGTGGGTGGGATGGGTGGGAGT-5′
13. 20/42-mer G4 DNA
5′-/FAM/-TTTGCCTCGAGCCAGCCGCAGAC-3′
    3′-CGGAGCTCGGTCGGCGTCTGCGTGGGTGGGATGGGTGGGAGT-5′
14. 21/42-mer G4 DNA
5′-/FAM/-TTTGCCTCGAGCCAGCCGCAGACG-3′
    3′-CGGAGCTCGGTCGGCGTCTGCGTGGGTGGGATGGGTGGGAGT-5′
15. 22/42-mer G4 DNA
5′-/FAM/-TTTGCCTCGAGCCAGCCGCAGACGC-3′
    3′-CGGAGCTCGGTCGGCGTCTGCGTGGGTGGGATGGGTGGGAGT-5′
16. 23/42-mer G4 DNA
5′-/FAM/-TTTGCCTCGAGCCAGCCGCAGACGCA-3′
    3′-CGGAGCTCGGTCGGCGTCTGCGTGGGTGGGATGGGTGGGAGT-5′
a

Tetrad-associated guanines are shown in bold with the G22 guanine underlined.