(a) Time needed to achieve one decimal reduction at 100 °C of spores of 18 strains of B. subtilis (assessed for two independent spore crops). On the basis of heat resistance of spores, strains belonged to one of two significantly different groups. One group contained nine strains with low-level heat-resistant spores (168, B4055, B4056, B4057, B4058, B4059, B4060, B4061, B4143), the other group contained nine strains with high-level heat-resistant spores (B4067, B4068, B4069, B4070, B4071. B4072, B4073, B4145, B4146). (b) Overview of the Tn1546-like transposon exclusively present in B. subtilis strains producing high-level heat-resistant spores.