Table 1.
Distance to LN2 surface | Cooling rate (°C/min) | Sperm motility (%) | Acrosomal integrity (%) |
1 cm | −435 | 9.7 ± 5.0b | 86.0 ± 1.9b |
4 cm | −183 | 25.0 ± 8.4c | 88.2 ± 1.7b |
7 cm | −69 | 18.2 ± 9.9c | 86.8 ± 2.0b |
Fresh sperm | — | 84.2 ± 11.3a | 91.7 ± 1.6a |
All samples were held in LN2 for 10 min. Different superscripted letters within a column indicate significant (P< 0.05) differences between values.