Table 1.
Effect of injection route on anesthetic parameters (mean ± SEM) in mice
| Sex | Route | n | Weight (g) | Died | Time to onset of immobilization (min) | Achieved surgical anesthesiaa | Time to onset of surgical anesthesia (min) | Duration of surgical anesthesia (min) | Duration of immobilization (min) | |
| BALB/c | ||||||||||
| F | IP | 10 | 20.1 ± 1.1 | 5 (50) | 2.9 ± 0.6 | 5/5 (100) | 6.7 ± 2.6 | 34.0 ± 5.5 | 67.0 ± 12.4 | |
| SC | 10 | 19.8 ± 1.3 | 0 | 2.8 ± 0.4 | 8/10 (80) | 12.5 ± 4.6 | 29.4 ± 9.0 | 67.8 ± 12.0 | ||
| M | IP | 10 | 24.8 ± 1.7 | 1(10) | 3.4 ± 0.5 | 8/9 (89) | 13.8 ± 4.4 | 28.8 ± 11.6 | 68.8 ± 14.2 | |
| SC | 10 | 25.0 ± 1.6 | 0 | 3.1 ± 0.9 | 8/10 (80) | 16.9 ± 8.0 | 25.0 ± 15.1 | 74.3 ± 16.1 | ||
| ICR | ||||||||||
| F | IP | 10 | 26.4 ± 1.9 | 2 (20) | 3.0 ± 0.5 | 2/8 (25) | 20.0 ± 10.0 | 15.0 ± 0.0 | 46.9 ± 6.3 | |
| SC | 10 | 24.7 ± 3.3 | 0 | 3.1 ± 0.7 | 6/10 (60) | 20.0 ± 7.8 | 17.5 ± 8.2 | 53.0 ± 8.0 | ||
| M | IP | 10 | 30.1 ± 1.9 | 0 | 2.7 ± 0.5 | 9/10 (90) | 6.7 ± 5.0 | 30.0 ± 7.1 | 51.7 ± 5.2 | |
| SC | 10 | 29.8 ± 1.8 | 0 | 2.4 ± 0.5 | 9/10 (90) | 15.6 ± 6.4 | 26.7 ± 12.0 | 59.1 ± 5.5 | ||
| C57BL/6J | ||||||||||
| F | IP | 10 | 18.0 ± 1.2 | 2 (20) | 2.4 ± 0.5 | 8/8 (100) | 15.0 ± 6.9 | 25.0 ± 11.0 | 72.6 ± 10.3 | |
| SCb | 9 | 19.1 ± 1.4 | 0 | 2.9 ± 0.3 | 6/9 (67) | 25.0 ± 6.7 | 27.5 ± 7.6 | 70.2 ± 19.6 | ||
| M | IP | 10 | 20.4 ± 1.3 | 0 | 2.3 ± 0.5 | 10/10(100) | 10.0 ± 7.8 | 30.5 ± 16.1 | 70.6 ± 9.1 | |
| SC | 10 | 21.0 ± 1.3 | 0 | 2.5 ± 0.5 | 8/10 (80) | 19.4 ± 15.0 | 25.6 ± 10.2 | 73.6 ± 10.4 | ||
| Total | ||||||||||
| F | IP | 30 | 21.4 ± 3.9 | 9 (30) | 2.8 ± 0.6 | 15/21 (71) | 14.4 ± 8.1 | 26.7 ± 10.5 | 61.5 ± 15.0 | |
| SCb | 29 | 21.4 ± 3.4 | 0 | 2.9 ± 0.5 | 20/29 (69) | 16.8 ± 6.9 | 25.3 ± 9.5 | 63.5 ± 15.4 | ||
| M | IP | 30 | 25.1 ± 4.3 | 1 (3) | 2.8 ± 0.6 | 27/29 (93) | 12.3 ± 6.7 | 29.8 ± 11.9 | 63.5 ± 13.0 | |
| SC | 30 | 25.3 ± 3.9 | 0 | 2.7 ± 0.7 | 25/30 (83) | 17.2 ± 6.5 | 21.5 ± 14.7 | 69.0 ± 13.2 | ||
| all | IP | 60 | 23.3 ± 4.5 | 10 (17) | 2.8 ± 0.6 | 42/50 (84) | 13.0 ± 7.3 | 28.0 ± 12.1 | 62.7 ± 13.8 | |
| SC | 59 | 23.4 ± 4.2 | 0 | 2.8 ± 0.6 | 45/59 (76) | 17.0 ± 6.6 | 25.6 ± 10.9 | 66.3 ± 14.5 | ||
F, female; IP, intraperitoneal; M, male; SC, subcutaneous
Continuous variables are displayed as mean ± 1 SD, and categorical variables are displayed as n (%).
a
Mice that died were excluded from analysis. Data are given as no. anesthetized/no. injected (% anesthetized)
b
One female C57BL/6J was excluded from the experiment due to low body weight.