Table 2. Analysis of molecular variance (AMOVA) results for biotype B and biotype Q populations.
Source of variation | dfa | SS | MS | Estimate of variance | % of total variation | Φ-Statistics | |
---|---|---|---|---|---|---|---|
Biotype B | Among populations / countries (within B biotype) | 6 | 209.094 | 34.849 | 1.714 | 27% | ΦPT = 0.271* |
Among individuals (within populations) | 125 | 575.315 | 4.603 | 4.603 | 73% | ||
Total | 131 | 784.409 | 6.317 | 100% | |||
Biotype Q | Among populations / countries (within Q biotype) | 8 | 791.194 | 98.899 | 4.031 | 46% | ΦPT = 0.458* |
Among individuals (within populations) | 204 | 972.360 | 4.766 | 4.766 | 54% | ||
Total | 212 | 1763.554 | 8.798 | 100% | |||
Biotype Q–Western Mediterranean | Among populations / countries (within Q biotype West Med) | 4 | 125.827 | 31.457 | 1.270 | 20% | ΦPT = 0.198* |
Among individuals (within populations) | 103 | 529.766 | 5.143 | 5.143 | 80% | ||
Total | 107 | 655.593 | 6.413 | 100% | |||
Biotype Q–Eastern Mediterranean | Among populations / countries (within Q biotype East Med) | 3 | 230.158 | 76.719 | 2.794 | 39% | ΦPT = 0.389* |
Among individuals (within populations) | 101 | 442.594 | 4.382 | 4.382 | 61% | ||
Total | 104 | 672.752 | 7.177 | 100% |
Estimates of ΦPT were calculated using 9 loci (excluding WF2C01, BEM6, BT-e49, BtIs1.2) on 132 samples from seven populations for biotype B and 11 loci (excluding BEM6, BT-b103) on 213 samples from 9 populations for biotype Q. Analyses within biotype Q were done on relevant subsets of the dataset, for Western and Eastern Mediterranean populations.
adf: degrees of freedom, SS: sum of squares, MS: mean squares
*significant at P<0.0001 (based on 9999 permutations)