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. 2016 Nov 11;5:99–113. doi: 10.2147/OV.S112107

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© 2016 Bhatia et al. This work is published and licensed by Dove Medical Press Limited

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Binding specificity of the bispecific adapter (sCAR-CXCL12).

Notes: Purified sCAR-CXCL12 was tested in ELISA against recombinant Ad5 fiber knob (Ad5 knob) and recombinant Ad3 fiber knob (Ad3 knob). Each antigen was absorbed at 300 ng/well. The wells were incubated with dilutions of the adapter protein. The binding was detected by treating the wells with an anti-His tag antibody followed by an anti-rabbit IgG antibody conjugated with HRP and color reaction with TMB substrate. The color intensity was measured as optical density at 450 nm. Numbers indicate the mean ± SEM of absorbance from three replicate dishes. The concentration results were compared using a Student’s t-test; the differences were considered statistically significant (*) if p<0.05.

Abbreviation: Ad, adenovirus; Ad3, Ad serotype 3; Ad5, Ad serotype 5; ELISA, enzyme-linked immunosorbent assay; His, histidine; HRP, horseradish peroxidase; sCAR, soluble ectodomain form of the native Ad5 receptor; SEM, standard error of the mean; TMB, 3,3′,5,5′-tetramethylbenzidine.