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. 2016 Nov 11;5:99–113. doi: 10.2147/OV.S112107

Figure 4.

Figure 4

Infection of a cancer cell line by an Ad pre-complexed with a bispecific adapter, sCAR-CXCL12, is concentration and time dependent.

Notes: After an initial 4 h infection with 100 ifu/cell Ad5-CMV-GFP-luc alone or pre-complexed with increasing concentrations of sCAR-CXCL12, the percent of MDA-MB-435S cells positive for GFP expression was quantified at 48 h by flow cytometry analysis (A). After infection with 100 ifu/cell Ad5-CMV-GFP-luc alone or pre-complexed with 100 ng/108 ifu of sCAR-CXCL12 for 5, 15, 30, 60, 120, and 240 min, the percent of MDA-MB-435S cells positive for GFP expression was quantified by flow cytometry analysis (B). Numbers indicate the mean ± SEM of percent GFP-positive cells from three replicate dishes.

Abbreviations: Ad, adenovirus; Ad5, Ad serotype 5; GFP, green fluorescent protein; ifu, infectious unit; luc, luciferase; sCAR, soluble ectodomain form of the native Ad5 receptor; SEM, standard error of the mean.