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. 2016 Nov 11;5:99–113. doi: 10.2147/OV.S112107

Figure 6.

Figure 6

Ablation of native liver tropism of Ad using a bispecific adapter (sCAR-CXCL12).

Notes: Luc assay at 48 h after infection of primary human liver slices with Ad5-CMV-GFP-luc in the absence or presence of 100 ng/108 ifu sCAR-CXCL12. (A) Mean RLU/μg protein ± SEM of three replicate dishes. Real-time PCR assay for the Ad5 E4 gene in extracts from human primary liver slices with Ad5-WT in the absence or presence of 100 ng/108 ifu sCAR-CXCL12 at 2 and 4 days after infection. (B) Mean E4 copy number ± SEM in extracts from three replicate dishes. The treatment results were compared using a Student’s t-test; the differences were considered statistically significant (*) if p<0.05.

Abbreviations: Ad, adenovirus; Ad5, Ad serotype 5; GFP, green fluorescent protein; ifu, infectious unit; luc, luciferase; PCR, polymerase chain reaction; sCAR, soluble ectodomain form of the native Ad5 receptor; SEM, standard error of the mean; WT, wild type; RLU, relative light unit.