Figure 8.
Determination of Ad targeting to CXCR4 by sCAR-CXCL12 in tumorigenic and immortalized non-tumorigenic cells.
Notes: Tumorigenic BT-20 (A), MCF-7 (B), and ZR-75-1 (C) cancer cells or non-tumorigenic MCF-12A epithelial cells (D) were plated at 1 × 105 cells/well in a 24-well plate. Indicated amounts of Ad5-CMV-GFP-luc pre-complexed in the absence or presence of sCAR-CXCL12 (100 ng/well) were incubated for 4 h at 37°C followed by a medium change. At 48 h post infection, the percentage of GFP-positive cells was determined by flow cytometry. Numbers indicate the mean ± SEM of percent GFP-positive cells from three replicate dishes. The treatment results were compared using a Student’s t-test; the differences were considered statistically significant (*) if p<0.05.
Abbreviations: Ad, adenovirus; Ad5, Ad serotype 5; GFP, green fluorescent protein; luc, luciferase; MOI, multiplicity of infection; sCAR, soluble ectodomain form of the native Ad5 receptor; SEM, standard error of the mean.