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. 2016 Nov 17;11(11):e0166591. doi: 10.1371/journal.pone.0166591

Fig 1. Identification of a novel isoform of 5-LO.

Fig 1

(A) Schematic representation of 5-LO WT and 5-LOΔ4 mRNA transcripts. The forward primer for 5-LO detection binds on the exon3-exon5 junction with 15 bp on exon 3 and 5 bp on exon 5. (B) Transcript expression profile of 5-LO WT and 5-LOΔ4 in Raji using PP1. Primer annealing temperature for 5-LO was 63°C, elongation time was 60 sec, 32 cycles were applied. (C) mRNA expression profile of 5-LO WT and 5-LOΔ4 in Raji, BL41, primary B cells and primary T cells using PP2. Primer annealing temperature for 5-LO was 54°C (Raji, B cells, T cells) or 56°C (BL41), elongation time was 60 sec, 33 cycles. The plasmids pcDNA3.1-5-LO WT and pcDNA3.1-5-LOΔ4 were used as positive control. Primer annealing temperature for B2M was 61°C, 22 cycles and the plasmid pCMV6-XL5–B2M was used as a positive control.