Fig 5. Influence of Δ4 isoform on the activity of 5-LO WT.

1 or 3 μg of 5-LO WT with or without 3 μg of Δ4 isoform were stimulated by 20 μM AA for 10 min at 37°C in the presence of 1 mM ATP with or without (w/o) addition of 2 mM calcium chloride (Ca) and/or 100 μg/ml PC (CaPC) in a total volume of 1 ml PBS/1 mM EDTA. The total protein content of each sample was adjusted to 30 μg/ml using γ-globulin. The formed 5-LO products were analyzed by HPLC and comprise 5-H(p)ETE and the all-trans-isomers of LTB₄. Data are shown as mean + standard error (SE) (n = 3), statistical analysis was conducted using an unpaired, two-tailored student’s t-test, p≤0.05 (*).