Table 1. Thermal stability of 5-LO WT and 5-LOΔ4 in absence or presence of 1 mM ATP or 1.5 mM calcium chloride.
Tm w/o [°C] | Tm + 1 mM ATP [°C] | Tm + 1.5 mM Ca [°C] | |
---|---|---|---|
C2ld WT | 42.6 + 3.9 | 48.9 + 0.7 | 44.5 + 0.7 |
Cat WT | 63.0 + 0.7 | 64.2 + 0.4 | 61.2 + 0.7 |
C2ld Δ4 | 39.3 + 1.5 | 43.0 + 0.7 | 40.9 + 1.8 |
Cat Δ4 | 47.3 + 0.3 | 50.2 + 0.1 | 47.3 + 0.1 |