Table 1. Thermal stability of 5-LO WT and 5-LOΔ4 in absence or presence of 1 mM ATP or 1.5 mM calcium chloride.

DSF assay conditions: 5x SYPRO^® orange; sample volume 50 μl; continuous heating 1.5°C/min, 15–80°C; melting temperatures T_mΔT_mΔT_m: n = 3, mean ±SEM. C2ld: C2-like domain, cat: catalytic domain. Data are shown as mean + standard error (SE) (n = 3).

	Tm w/o [°C]	Tm + 1 mM ATP [°C]	Tm + 1.5 mM Ca [°C]
C2ld WT	42.6 + 3.9	48.9 + 0.7	44.5 + 0.7
Cat WT	63.0 + 0.7	64.2 + 0.4	61.2 + 0.7
C2ld Δ4	39.3 + 1.5	43.0 + 0.7	40.9 + 1.8
Cat Δ4	47.3 + 0.3	50.2 + 0.1	47.3 + 0.1