Fig 7. Neutrophil infiltration goes back to normal levels after clearance of the double mutant ΔvraGΔhemB (ΔΔ) from the mammary glands.

Mice were infected as described in materials and methods, and a non-infected control group of mice received a sterile PBS injection (PBS). Glands were harvested at the indicated times, homogenized and kinetically assayed for MPO activity as described in materials and methods. Columns represent means of MPO Units of a group of 6 glands (4 for the PBS control) adjusted by gram of gland, and error bars illustrate standard deviation. Statistical significance between the Day 4 and 12 groups post infection is shown by the “Φ” symbols. One-Way ANOVA and Tukey’s multiple comparison tests were used (ΦΦ, P≤ 0.01; NS, no significant difference between groups).