Table 3. Genes categorized by protein function based on BLAST searches that were differentially expressed in SSH library B (NT under well-watered conditions compared to NT under drought stressed conditions) in the forward (up-regulated by drought) and reverse (down-regulated by drought) libraries.
Numbers in parentheses indicate number of transcript isoforms detected.
| Library | Description | Accession # | Best Match Accession | E-value |
|---|---|---|---|---|
| Category 01 Metabolism | ||||
| Forward | malate dehydrogenase | JZ948432 | XP_001659012.1 | 2.00E-29 |
| glycogen synthase kinase-3 MsK-3 | JZ948433 | NP_001148880.1 | 6.00E-07 | |
| Reverse | nitrilase-associated protein, putative | JZ948434 | CAJ38376.1 | 4.00E-12 |
| thiamine biosythesis protein ThiC | JZ948435 | AAG49550.1 | 1.00E-54 | |
| Category 02 Energy | ||||
| Forward | RuBisCO large subunit (3) | JZ948436 | BAD20627.1 | 2.00E-44 |
| JZ948437 | BAD20627.1 | 8.00E-60 | ||
| JZ948438 | BAD20627.1 | 1.00E-53 | ||
| RuBisCO small subunit 1B | JZ948439 | NP_198659.1 | 0.00001 | |
| RuBisCO small chain c | JZ948440 | ABR26034.1 | 0.00013 | |
| Oxygen-evolving enhancer protein 3–1, chloroplast precursor | JZ948441 | BAC83128.1 | 1.00E-37 | |
| cytochrome c oxidase subunit III | JZ948442 | YP_003734710.1 | 8.00E-36 | |
| Photosystem II 10 kDa polypeptide, chloroplast | JZ948443 | NP_001134061.1 | 4.00E-60 | |
| thioredoxin-like 5, chloroplastic | JZ948444 | ABR26107.1 | 3.00E-30 | |
| ATP-citrate synthase, putative | JZ948445 | XP_002519229.1 | 5.00E-49 | |
| Reverse | RuBisCO large subunit (3) | JZ948446 | ADU18941.1 | 2.00E-76 |
| JZ948447 | ADU18941.1 | 2.00E-15 | ||
| JZ948448 | ADU18941.1 | 3.00E-08 | ||
| RuBisCO | JZ948448 | CBF07493.1 | 1.00E-26 | |
| glyceraldehyde-3-phosphate dehydrogenase 1 | JZ948450 | ACV86034.1 | 8.00E-107 | |
| oxygen-evolving enhancer protein 1, chloroplast | JZ948451 | ABQ52657.1 | 9.00E-20 | |
| Category 05: Protein synthesis | ||||
| Forward | elongation factor 1 alpha | JZ948417 | AEG78681.1 | 2.00E-112 |
| Category 06: Protein destination/storage | ||||
| Forward | E3 ubiquitin-protein ligase UBR5 | JZ948540 | EFN82877.1 | 2.00E-57 |
| thiol disulfide interchange protein txlA | JZ948453 | NP_001152226.1 | 2.00E-06 | |
| GTPase SAR1 | JZ948452 | ACD03831.1 | 3.00E-57 | |
| Reverse | ubiquitin-60S ribosomal protein L40-like | JZ948454 | XP_003465244.1 | 1.00E-12 |
| ubiquitin-conjugating enzyme E2-like protein | JZ948455 | ADB28900.1 | 2.00E-14 | |
| ATP-dependent Clp protease ATP-binding subunit clpA | JZ948456 | P31542.1 | 9.00E-34 | |
| Category 07 Transporters | ||||
| Forward | vacuolar H+-ATPase subunit B | JZ948457 | BAF38479.1 | 2.00E-61 |
| ABC transporter integral membrane protein | JZ948458 | ZP_07303354.1 | 0.00061 | |
| CDGSH iron sulfur domain 1 | JZ948459 | NP_001004811.1 | ||
| Category 10 Signal transduction | ||||
| Forward | uridine kinase | JZ948460 | ZP_00781761.1 | 0.00042 |
| serine/threonine kinase receptor precursor-like | JZ948461 | BAC57306.1 | 4.00E-39 | |
| Reverse | LRR receptor-like kinase (3) | JZ948462 | ACY30448.1 | 7.00E-70 |
| Category 11 Disease/Stress defense | ||||
| Forward | jasmonate-induced protein, putative | JZ948463 | ABA96835.1 | 3.00E-08 |
| universal stress protein 5327 | JZ948464 | ADB54812.1 | 4.00E-37 | |
| abscisic acid-responsive HVA22 family protein | JZ948465 | XP_002865508.1 | 3.00E-08 | |
| glyoxalase I | JZ948466 | AAW68026.1 | 1.00E-53 | |
| Category 20 Secondary metabolism | ||||
| Reverse | diphthine synthase, predicted | JZ948467 | XP_001604120.1 | 6.00E-75 |
| Category 12 Unclear | ||||
| Forward | predicted protein [Hordeum vulgare subsp. vulgare] | JZ948468 | BAK02049.1 | 1.00E-23 |
| predicted protein [Hordeum vulgare subsp. vulgare] | JZ948469 | BAJ85089.1 | 3.00E-42 | |
| predicted protein [Hordeum vulgare subsp. vulgare] | JZ948470 | BAJ96581.1 | 3.00E-21 | |
| SORBIDRAFT_10g001310 | JZ948471 | XP_002436374.1 | 0.00002 | |
| pBMB0558_00760 | JZ948472 | YP_004169259.1 | 5.00E-04 | |
| predicted protein [Hordeum vulgare subsp. vulgare] | JZ948473 | BAJ95019.1 | 1.00E-32 | |
| predicted protein [Hordeum vulgare subsp. vulgare] | JZ948474 | BAJ99280.1 | 5.00E-12 | |
| hypothetical protein MELLADRAFT_95019 | JZ948475 | EGF98952.1 | 1.00E-23 | |
| predicted protein [Hordeum vulgare subsp. vulgare] | JZ948476 | BAJ90401.1 | 3.00E-16 | |
| hypothetical protein OsJ_26652 | JZ948477 | EAZ42091.1 | 6.00E-17 | |
| Reverse | predicted protein | JZ948478 | XP_001770883.1 | 0.0004 |
| hypothetical protein OsJ_19720 | JZ948479 | EEE64863.1 | 1.00E-57 | |
| hypothetical protein Tc00.1047053508475.20 | JZ948480 | XP_804280.1 | 0.000031 | |
| hypothetical protein LOC100273563 | JZ948481 | NP_001141453.1 | 8.00E-12 | |