Table 5. Genes categorized by protein function based on BLAST searches that were differentially expressed in SSH library D, which compared NT to SAG12-ipt under drought stressed conditions equal in cellular water deficit (47% RWC) in the forward (up-regulated by transgene during drought stress) and reverse (down-regulated by transgene during drought stress) libraries.
Numbers in parentheses indicate number of transcript isoforms detected.
| Library | Description | Accession # | Best Match # | E value |
|---|---|---|---|---|
| Category 01 Metabolism | ||||
| Forward | GDP-mannose 3,5-epimerase | JZ948450 | XM_003577361.1 | 3.00E-27 |
| mannose-1-phosphate guanylyltransferase 3 | JZ948505 | Q6Z9A3.1 | 3.00E-149 | |
| UDP-arabinopyranose mutase 1 | JZ948506 | Q6Z9A3.1 | 1.00E-15 | |
| glycosyl hydrolase family 19 protein | JZ948507 | AAQ84319.1 | 6.00E-44 | |
| UTP—glucose-1-phosphate uridylyltransferase | JZ948508 | Q43772.1 | 1.00E-15 | |
| UDP-glucose dehydrogenase | JZ948509 | AAX08057.1 | 7.00E-25 | |
| xylose isomerase-like | JZ948510 | XM_003562448.1 | 4.00E-23 | |
| xylose isomerase | JZ948511 | CAA64544.1 | 3.00E-25 | |
| Reverse | glycine decarboxylase P subunit | JZ948512 | AAB82711.1 | 8.00E-12 |
| acetyl-CoA carboxylase | JZ948513 | NP_001185143.1 | 4.00E-21 | |
| Category 02 Energy | ||||
| Forward | RuBisCo large subunit (3) | JZ948514 | AAQ08331.1 | 5.00E-61 |
| JZ948515 | AAQ08331.1 | 1.00E-26 | ||
| JZ948538 | AAQ08331.1 | 2.00E-06 | ||
| chloroplast-localized Ptr ToxA-binding protein1 | JZ948539 | AAR24582.1 | 6.00E-54 | |
| Reverse | RuBisCo large subunit (4) | JZ948516 | ACO35581.1 | 4.00E-46 |
| JZ948517 | ACO35581.1 | 5.00E-61 | ||
| JZ948518 | ACO35581.1 | 2.00E-17 | ||
| JZ948519 | ACO35581.1 | 3.00E-76 | ||
| thioredoxin-like 5, chloroplastic | JZ948484 | ABR26107.1 | 3.00E-30 | |
| ATP-citrate synthase, putative | JZ948445 | XP_002519229.1 | 5.00E-49 | |
| Category 04 Transcription | ||||
| Forward | partial 16S rRNA gene | JZ948520 | FN421445.1 | 2E-15 |
| histone H3 | JZ948521 | XP_001752178.1 | 1.00E-121 | |
| translational initiation factor eIF1 | JZ948522 | BAF63490.1 | 2.00E-11 | |
| Category 05 Protein synthesis | ||||
| Forward | Os07g0609766 | JZ948523 | NP_001175295.1 | 3.00E-05 |
| Category 07 Transporters | ||||
| Forward | aquaporin PIP1-2 | JZ948524 | NP_001078067.1 | 1.00E-32 |
| coatomer subunit beta'-2 | JZ948525 | NP_175645.1 | 1.00E-49 | |
| Reverse | plasma membrane H+-ATPase | JZ948526 | CAC50884.1 | 1.00E-28 |
| Category 10 Signal transduction | ||||
| Forward | receptor kinase ORK14 | JZ948527 | AAM09948.1 | 1.00E-5.8 |
| Category 11 Disease/Stress defense | ||||
| Forward | Chloroplast Ptr ToxA-binding protein | JZ948529 | AK332987.1 | 1.00E-45 |
| glyoxalase I | JZ948528 | BAB71741.1 | 9.00E-26 | |
| Category 20 Secondary metabolism | ||||
| Forward | cruciferin cru4 subunit | JZ948530 | X57848.1 | 2.00E-11 |
| isoflavone reductase | JZ948531 | ACH72670.1 | 4.00E-57 | |
| Category 12 Unclear | ||||
| Forward | Os11g0169100 | JZ948532 | NP_001065849.1 | 7.00E-50 |
| hypothetical protein | JZ948533 | ACG32534.1 | 5.00E-13 | |
| predicted protein | JZ948534 | BAK07943.1 | 1.00E-41 | |
| predicted protein | JZ948536 | BAK05471.1 | 5.00E-06 | |
| Reverse | hypothetical protein | JZ948537 | CAM36311.1 | 8.00E-07 |