Fig. 6.
Mitochondrial H2S donors affect the cellular bioenergetics. b.End3 cells exposed to 7-day-long hyperglycemia were treated with AP39 (30 nM) or AP123 (100 nM) and the metabolic profile of the cells was studied by extracellular flux analysis. Sequential injections of Oligomycin (1 μg/ml), FCCP (0.3 μM) and antimycin A (2 μg/ml) were used to measure A: the cellular oxygen consumption rate (OCR) and B: the extracellular acidification rate (ECAR). C: Basal oxygen consumption, D: ATP production linked oxygen consumption (determined by oligomycin injection), E: total respiratory capacity (determined following the addition of FCCP) and F: the proton leak/basal respiration was determined. G: Acid production of basal metabolism and H: acid production during anaerobic compensation was determined. AP39 and AP123 increase the respiratory capacity of the cells. (n = 3, *p < 0.05 compared to hyperglycemic control).