Pressure-area profiles of the mixtures of sebum with meibum on the artificial tear solution at 35°C. (A) Profiles with surface area. (B) Profiles with molecular area. Data show compression parts of the profiles of sebum:meibum mixtures (S:M in mole fraction ratios 0.25:0.75, 0.70:0.30, and 0.97:0.03) and meibum (M). Sebum increased the average molecular area in the 0.25:0.75 mixture, did not affect it much in the 0.70:0.30 mixture, and decreased it in the 0.97:0.03 mixture, in comparison with the molecular area of meibum alone.