Figure 4. dsx/glutamatergic neurons are poised for dsx/GABAergic inhibition.

(a–a2) Overlay of expression of (a1) dsx/Gad1 presynaptic boutons and dsx/vGlut dendrites on standardized template VNC. (a1) dsx/Gad1 presynaptic boutons visualized by dsx^GAL4-DBD/Gad1^p65-AD driving UAS-nSyb::GFP in male VNC stained with anti-GFP (green). (a2) dsx/vGlut dendrites visualized by vGlut^dVP16-AD/dsx^GAL4-DBD driving UAS-DenMark in male VNC stained with anti-DsRed (magenta); neuropil counterstained with anti-nC82 (blue). (a–a2) Maximum intensity z-stacks and (a’–a2’) single section images. Solid arrowheads point at regions of close proximity. Scale bar = 50 μm. See also Video 5. (b) Effects of knocking down GABA_B-R2 receptor by RNAi in dsx/vGlut neurons on male copulatory behavior. Percentage of males displaying a ‘stuck’ phenotype is graphed (n = 20–30). *p<0.05 by Fisher exact test.

DOI: http://dx.doi.org/10.7554/eLife.20713.015

Figure 4—figure supplement 1. Effects of knocking down GABA receptor subunits in dsx/glutamatergic neurons on copulatory behaviors.

(a) Percentage of successful matings in 1 hr (n = 24–30). (b) Copulation duration (n = 12–24). (c) Male fertility (n = 30). Genotypes indicate males. Statistical comparisons of the experimental genotype were made against controls of the same receptor. (d) dsx/Gad1 neurons have prolific dendrites in regions of the Abg occupied by genital sensory terminals. dsx/Gad1 dendrites visualized by dsx^GAL4-DBD/Gad1^p65-AD driving UAS-DenMark in male VNC. Maximum intensity z-projection (d) and higher magnification image of 10 μm sub-stack (d’) is shown. DenMark stained with anti-dsRed (green); neuropil counterstained with anti-nC82 (magenta). Scale bar = 50 μm.

DOI: http://dx.doi.org/10.7554/eLife.20713.016