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. Author manuscript; available in PMC: 2016 Nov 17.
Published in final edited form as: Nat Immunol. 2010 Mar 14;11(4):295–302. doi: 10.1038/ni.1855

Figure 6.

Figure 6

Inhibition of iNKT cell cluster formation, average crawling velocity and stationary adhesion by pertussis toxin, anti-CXCR3 and anti-CD1d. Analysis of the hepatic microvasculature (visualized by spinning-disk confocal intravital microscopy) and formation of iNKT cell clusters (counted by intravital video) in Cxcr6gfp/+ mice pretreated with pertussis toxin (PTX), anti-CXCR3, anti-CD1d and/or α-GalCer (α-GC) before injection of B. burgdorferi. (a) Distribution of iNKT cells after PTX treatment, assessed 24 h after infection. (b) Cluster formation by B. burgdorferi at 24 h after infection. Original magnification, ×4 (a,b). (c,d) Effect of pretreatment on the average crawling velocity (c) and stationary arrest (d) of iNKT cells at 24 h after infection. (e) Liver iNKT cells during the first 24 h of B. burgdorferi infection. (f) Effect of pretreatment on the number of iNKT cells in the liver sinusoids (n = 4–6 mice per group). Original magnification, ×10 (e,f). P values (b–d), Bonferroni’s multiple-comparison test. Data are representative of three experiments (a) or more than two independent experiments per group (b–f; error bars, s.e.m.).