Figure 2.

Creation of virtual model of physical tissue sample on virtual 3D-printed IA model and alignment to 3D vascular model. A) pre-operative 3D image, B) reconstruction of vascular model from 3D image, C) ChenPen3D image, with clinical features noted as explained in Figure 1, D) introduction of small extrusions visible in the 3D-printed model for later alignment (red and blue arrows point to extrusions corresponding to red and blue markings in C, white arrow points to small incision made to reference MPM imaging regions), E) 3D-printed aneurysm model with metallic coating, F) tissue sample placed on 3D-printed IA aligned using markings on tissue and small extrusions, G) wrapping to prevent dehydration, H) 2D image of micro-CT scan of tissue sample mounted on 3D-printed aneurysm shown in G, I) reconstruction of combined virtual model of 3D-printed IA and tissue sample from micro-CT data, J) segmentation of virtual 3D-printed aneurysm, K) segmentation of virtual tissue sample, L) alignment of virtual 3D-printed IA back to 3D vascular model showing alignment of extruded features, and M) final alignment of virtual tissue sample to original vascular model (B) using prior alignments in (I) and (L).