Table 5.
β-galactosidase activities of NGR234 and NGR234-ΔtraI-ΔngrI carrying either a nodABC::lacZ or a nopB::lacZ promoter fusion and in response to apigenin.
| Rhizobium (construct) | Time Period (hours) | ||||
|---|---|---|---|---|---|
| 24 | 48 | 72 | 96 | 120 | |
| NGR234 (pnodABC) | 79.32±9.9 | 55.45±2.41 | 11.75±1.87 | 13.08±0.59 | 5.97±0.64 |
| NGR234 (pnodABC) + apigenin | 1597.05±170.32 | 1518.06±101.32 | 907.16±212.13 | 785.80±25.78 | 528.24±232.87 |
| NGR234 (pnopB) | 146.12±0.77 | 54.98±8.49 | 11.46±0.75 | 9.87±0.04 | 2.50±0.20 |
| NGR234 (pnodB) + apigenin | 1238.19±60.35 | 1086.54±51.51 | 86.98±55.71 | 84.75±2.21 | 5.23±0.0 |
| ngrIΔ/traIΔ (pnodABC) | 147.07±2.00 | 92.18±2.02 | 53.21±05.00 | 71.02±0.67 | 47.35±0.45 |
| ngrIΔ/traIΔ (pnodABC) + apigenin | 2430.34±253.73 | 3090.85±35.53 | 2299.20±1.74 | 2026.67±16.51 | 1952.91±0.41 |
| ngrIΔ/traIΔ (pnopB | 125.97±16.48 | 119.69±0.41 | 65.22±5.52 | 62.2±3.06 | 52.12±0.91 |
| ngrIΔ/traIΔ (pnodB) + apigenin | 670.58±199.42 | 2684.47±6.83 | 2023.53±29.54 | 1835.47±8.95 | 1733.38±19.18 |
Data are mean values with the corresponding standard deviation of 3 measurements. Promoters fused to the ß-galactiosdase gene are given in brackets as pnodABC or pnopB.