Figure 3.

Skeletal muscle gene and protein expression of mitochondrial complex subunits in adult male offspring. (A) RNA was extracted and RT-PCR array technology was utilized to determine expression of NADH ubiquinone dehydrogenase 1β subcomplex 8 (Ndfub8), succinate dehydrogenase iron-sulfur subunit (Sdhb), Cytochrome b-c1 complex subunit 2 (Uqcrc2), mitochondrial encoded cyctochrome c oxidase I (Mt-co1), and ATP synthase α (Atp5a) (B) Soleus muscle protein was extracted and protein expression of NDUFB8, SDHB, UQCRC2, MT-CO1 and ATP5 was determine by western blot. Representative blots are shown. ^*p < 0.05, CD vs. HF; n = 5–6 litters/group. Data are expressed as means ± SEM.