Table 1.
Morphometrics, and skeletal muscle enzyme activity.
| CD Offspring | HF Offspring | P-Value | |
|---|---|---|---|
| Soleus weight (mg) | 259.3±13.0 | 264.38±11.8 | 0.774 |
| Soleus weight, % body weight | 0.40±0.02 | 0.372±0.02 | 0.178 |
| IRS-1 gene expression (relative to CD) | 1.0±0.09 | 0.76±0.05* | 0.039 |
| GLUT4 gene expression (relative to CD) | 1.0±0.11 | 0.69±0.06* | 0.033 |
| mtDNA/nDNA (relative to CD) | 1.0±0.07 | 0.85±0.06 | 0.112 |
| METABOLIC ENZYME ACTIVITY | |||
| Citrate synthase (nM/min/μg protein) | 2566.26±317.90 | 2891.19±308.25 | 0.474 |
| Lactate dehydrogenase (nM/min/μg protein) | 2261.62±247.69 | 2308.74±222.31 | 0.829 |
| Carnitine palmitoyl transferase-1 (nM/min/μg protein) | 20.78±1.14 | 31.07±2.98* | 0.004 |
| Hydroxyacyl-Coenzyme A dehydrogenase (nM/min/μg protein) | 188.60±23.21 | 214.04±19.27 | 0.271 |
Soleus muscle protein was extracted to analyse activity of metabolic enzymes (citrate synthase, lactate dehydrogenase, carnitine palmitoyl transferase 1, and HADH)
*
p < 0.05 CD vs. HF; n = 5–6 litters per group. Data are expressed as means ± SEM, relative to total protein yield.