Table 2.

RT-PCR array differentially expressed genes.

Complex	Symbol	GeneBank	Fold change (Relative to CD)	P-Value
Complex I (NADH ubiquinone oxidoreductase)	Ndufs7	NM_001008525	−1.98	0.002
	Ndufs3	NM_001106489	−1.8	< 0.001
	Ndufa8	NM_001047862	−1.78	0.001
	Ndufs1	NM_001005550	−1.62	0.009
	Ndufb5	NM_001106426	−1.55	0.012
	Ndufa5	NM_012985	−1.44	0.004
	Ndufb3	NM_001106912	−1.43	0.022
	Ndufb8	NM_001106360	−1.36	0.012
Complex II (Succinate dehydrogenase)	Sdha	NM_130428	−1.8	0.008
	Sdhc	NM_001005534	−1.29	0.013
Complex III (Coenzyme Q- cytochrome C reductase)	Cyc1	NM_001130491	−2.16	< 0.001
	Uqcrc2	NM_001006970	−1.49	0.012
Complex IV (Cytochrome C oxidase)	Cox6a2	NM_012812	−1.65	0.011
	Cox15	NM_001033699	−1.59	0.003
	Cox4i2	NM_053472	−1.57	0.004
	Cox4i1	NM_017202	−1.55	0.009
	Cox7b	NM_182819	−1.51	0.012
	Cox7a2l	NM_001106704	−1.4	0.024
Complex V (ATP Synthase)	Atp6ap1	NM_031785	−1.87	0.022
	Atp5b	NM_134364	−1.66	0.006
	Atp5g2	NM_133556	−1.55	0.004
	Atp5l	NM_212516	−1.44	0.024
	Atp5i	NM_080481	−1.38	0.006
	Atp5d	NM_139106	−1.38	0.020
	Atp5h	NM_019383	−1.35	0.001
Accessory Proteins	Ucp2	NM_019354	−3.1	0.001
	Ucp3	NM_013167	−1.71	0.009
	Lhpp	NM_001009706	−1.65	0.010
	Ppa1	NM_001100834	−1.48	0.016

RT-PCR array analysis identified 29 genes that were differentially expressed in HF offspring. Of the 29 genes that were down regulated, 8 genes encode for complex I (NADH ubiquinone oxioreductase), 2 encode for complex II (succinate dehydrogenase), 2 encode for complex III (Coenzyme Q-cytochrome C reductase), 6 encode for complex IV (cytochrome c oxidase), 7 encode for complex V (ATPsynthase), and 4 accessory genes that encode for proteins involved in oxidative metabolism. Colors reflect fold change intensity.