Table 1.

Probes and primers used for amplification of virulence genes in real-time PCR assays (ISO/TS 13136, 2012 and this study).

Oligo name	5′ -Label	Primer sequence <5′ → 3′>	3′-Label	Reference
stx1- Probe	FAM	CTG GAT GAT CTC AGT GGG CGT TCT TAT GTA A	BHQ1	ISO/TS 13136, 2012
stx1- Forward		TTT GTT ACT GTG ACA GCT GAA GCT TTA CG
stx1- Reverse		CCC CAG TTC AAT GTA AGA TCA ACA TC
eae- Probe	ROX	ATA GTC TCG CCA GTA TTC GCC ACC AAT ACC	BHQ2
eae- Forward		CAT TGA TCA GGA TTT TTC TGG TGA TA
eae- Reverse		CTC ATG CGG AAA TAG CCG TTA
stx2- Probe	FAM	CTG TCT GAA ACT GCT CCT GTG	BHQ1	This study
stx2- Forward		CCA GTT CAG AGT GAG GTC CA
stx2- Reverse		TCA GTT CGA TAC CCG CTG CAG C
hylA-Probe	ROX	TCT CCG GAA TTC TTT CTG CT	BHQ2	This study
hylA-Forward		GCG AAA CAG CTT TAC CAA CA
hylA-Reverse		CGTC TCC CGG CGTC ATC GTA