Table 1.
Names and sequences of primers used in EPSPS gene sequencing and expression analysis of L. virgata populations.
| Name | Sequence (5′ to 3′) |
|---|---|
| EPSPS gene sequencing and cloning | |
| Lv-F3a | AAGAGCTGTWGTYGTTGGCTG |
| Lv-R2a | AATAGCACCTCGCACTTGAG |
| M13F | CGCCAGGGTTTTCCCAGTCACGAC |
| M13R | TCACACAGGAAACAGCTATGAC |
| qPCR | |
| qLv-For1a | GGCAGGTTCCCGATTGARAA |
| qLv-Rev1a | YGCATTTCCACCAGCAGCTA |
| ADP-RF(a) For | TCTCATGGTTGGTCTCGATG |
| ADP-RF(a) Rev | GGATGGTGGTGACGATCTCT |
| β-Act F1a | ATGGTAGGGATGGGACAGAA |
| β-Act R1a | TCCATGTCATCCCAGTTGCT |
aThe primers were designed with the software Primers3Plus online.